Secretion là gì

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aGuangdong & Shenzhen Key Laboratory of Male Reproductive Medicine và Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, ChinabGraduate School, Anhui Medical University, Hefei, ChinacGraduate School, Guangzhou Medical University, Guangzhou, China


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Qiong Deng

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Futian District, Shenzhen, 518036 (China)

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Abstract

Aims: This study aimed lớn examine the physiological mechanism whereby the corticotropin-releasing hormone (CRH) và arginine vasopressin (AVP) exert their influence on adrenocorticotropic hormone (ACTH) secretion in pituitary cells. Methods: Anterior pituitary cells were harvested from male rats và placed in the perifusion system. Cells were perifused with serum-free medium for 6 hours before fraction collection. After 30-minute of baseline collection, perifusion truyền thông was changed to lớn expose the cells to CRH with or without AVP.. ACTH concentration in each fraction was measured using enzyme immunoassay chemiluminescent kit. Results: The lowest physiological concentration of CRH (10 pM) or AVP (10 pM) was not able to lớn induce a marked increase in ACTH secretion. Higher concentration of CRH (30 pM) or AVP (100 pM) in the physiological range caused sustained elevation of ACTH secretion (Phường < 0.001), while the secretion remained at similar levels for up khổng lồ 1 hour with continuous stimulation. Perifusion with 10 pM AVPhường and 10 pM or 30 pM CRH caused a 2.38-fold and 2.99-fold increase in pulsatile ACTH secretion in pituitary cells, respectively. The duration of pulsatility caused by perifusion with 10 pM AVP and 30 pM CRH was close khổng lồ that observed under physiological condition. Conclusions: By using the rat anterior pituitary cell perifusion system, we found that CRH & AVP potentiate the effect of each other on ACTH secretion, but AVPhường was a less potent agonist than CRH. The data suggest that CRH and AVP. are essential for the pulsatility of ACTH, & AVP acts like a switch of the pulsatility.


Aguilera G: Regulation of pituitary ACTH secretion during chronic bao tay. Front Neuroendocrinol 1994;15: 321-350.
Antoni FA: Hypothalamic control of adrenocorticotropin secretion: advances since the discovery of 41-residue corticotropin-releasing factor. Endocr Rev 1986;7: 351-378.
Kabir NN, Kazi JU: Grb10 is a dual regulator of receptor tyrosine kinase signaling. Mol Biol Rep 2014;41: 1985-1992.
Vale W, Rivier C, Brown MR, Spiess J, Koob G, Swanson L, Bilezikjian L, Bloom F, Rivier J: Chemical and biological characterization of corticotropin releasing factor. Recent Prog Horm Res 1983;39: 245-270.
Autelitano DJ, Lundblad JR, Blum M, Roberts JL: Hormonal regulation of POMC ren expression. Annu Rev Physiol 1989;51: 715-726.
Bale TL, Vale WW: CRF & CRF receptors: role in áp lực responsivity và other behaviors. Annu Rev Pharmacol Toxicol 2004;44: 525-557.
Abou-Samra AB, Harwood JPhường, Manganiello VC, Catt KJ, Aguilera G: Phorbol 12-myristate 13-acetate & vasopressin potentiate the effect of corticotropin-releasing factor on cyclic AMP. production in rat anterior pituitary cells. Mechanisms of action. J Biol Chem 1987;262: 1129-1136.
Gillies GE, Linton EA, Lowry PJ: Corticotropin releasing activity of the new CRF is potentiated several times by vasopressin. Nature 1982;299: 355-357.
Aguilera G, Pyêu thích Q, Rabadan-Diehl C: Regulation of pituitary vasopressin receptors during chronic stress: relationship to lớn corticotroph responsiveness. J Neuroendocrinol 1994;6: 299-304.
Levin N, Blum M, Roberts JL: Modulation of basal và corticotropin-releasing factor-stimulated proopiomelanocortin gen expression by vasopressin in rat anterior pituitary. Endocrinology 1989;125: 2957-2966.
Veldhuis JD, Iranmanesh A, Johnson ML, Lizarralde G: Amplitude, but not frequency, modulation of adrenocorticotropin secretory bursts gives rise to the nyctohemeral rhythm of the corticotropic axis in man. J Clin Endocrinol Metab 1990;71: 452-463.
Yamase K, Takahashi S, Nomura K, Haruta K, Kawashima S: Circadian changes in arginine vasopressin màn chơi in the suprachiasmatic nuclei in the rat. Neurosci Lett 1991;130: 255-258.
Jin X, Shearman LP, Weaver DR, Zylka MJ, de Vries GJ, Reppert SM: A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clochồng. Cell 1999;96: 57-68.
Plotsky PM, Vale W: Hemorrhage-induced secretion of corticotropin-releasing factor-like immunoreactivity inlớn the rat hypophysial portal circulation & its inhibition by glucocorticoids. Endocrinology 1984;114: 164-169.
Fink G, Robinson IC, Tannahill LA: Effects of adrenalectomy and glucocorticoids on the peptides CRF-41, AVPhường và oxytocin in rat hypophysial portal blood. J Physiol 1988;401: 329-345.
Antoni FA: Vasopressinergic control of pituitary adrenocorticotropin secretion comes of age. Front Neuroendocrinol 1993;14: 76-122.
Mc CS, Brobeông xã JR: Evidence for a role of the supraopticohypophyseal system in regulation of adrenocorticotrophin secretion. Proc Soc Exp Biol Med 1954;87: 318-324.
Whitnall MH: Regulation of the hypothalamic corticotropin-releasing hormone neurosecretory system. Prog Neurobiol 1993;40: 573-629.
de Goeij DC, Kvetnansky R, Whitnall MH, Jezova D, Berkenbosch F, Tilders FJ: Repeated stress-induced activation of corticotropin-releasing factor neurons enhances vasopressin stores and colocalization with corticotropin-releasing factor in the median eminence of rats. Neuroendocrinology 1991;53: 150-159.
Deng Q, Riquelme D, Trinc L, Low MJ, Tomic M, Stojilkovic S, Aguilera G: Rapid Glucocorticoid Feedback Inhibition of ACTH Secretion Involves Ligand-Dependent Membrane Association of Glucocorticoid Receptors. Endocrinology 2015;156: 3215-3227.
Prickett TCR, Inder WJ, Evans MJ, Donald RA: Interleukin-1 potentiates basal và AVP-stimulated ACTH secretion in vitro–the role of CRH pre-incubation. Horm Metab Res 2000;32: 350-354.
Walker JJ, Spiga F, Waite E, Zhao Z, Kershaw Y, Terry JR, Lightman SL: The origin of glucocorticoid hooc môn oscillations. PLoS Biol 2012;10:e1001341.
Engler D, Psi mê T, Liu JP.., Fullerton MJ, Clarke IJ, Funder JW: Studies of the regulation of the hypothalamic-pituitary-adrenal axis in sheep with hypothalamic-pituitary disconnection. II. Evidence for in vivo ultradian hypersecretion of proopiomelanocortin peptides by the isolated anterior và intermediate pituitary. Endocrinology 1990;127: 1956-1966.
Wersinger SR, Ginns EI, O"Carroll AM, Lolait SJ, Young WS, 3rd: Vasopressin V1b receptor knockout reduces aggressive sầu behavior in male mice. Mol Psychiatry 2002;7: 975-984.
Makara GB, Mergl Z, Zelena D: The role of vasopressin in hypothalamo-pituitary-adrenal axis activation during stress: an assessment of the evidence. Ann N Y Acad Sci 2004;1018: 151-161.
Tanoue A, Ilớn S, Hondomain authority K, Oshikawa S, Kitagawa Y, Koshimizu TA, Mori T, Tsujimolớn G: The vasopressin V1b receptor critically regulates hypothalamic-pituitary-adrenal axis activity under both bít tất tay và resting conditions. J Clin Invest 2004;113: 302-309.
Rivier J, Rivier C, Vale W: Synthetic competitive sầu antagonists of corticotropin-releasing factor: effect on ACTH secretion in the rat. Science 1984;224: 889-891.
Olsen L, Knigge U, Warberg J: Gastrin-releasing peptide stimulation of corticotropin secretion in male rats. Endocrinology 1992;130: 2710-2716.
Sudomain authority T, Nakano Y, Tozawa F, Sumitomo T, Sato Y, Yamadomain authority M, Demura H: The role of corticotropin-releasing factor và vasopressin in hypoglycemia-induced proopiomelanocortin gene expression in the rat anterior pituitary gland. Brain Res 1992;579: 303-308.
Jard S, Barberis C, Audigier S, Tribollet E: Neurohypophyseal hooc môn receptor systems in brain and periphery. Prog Brain Res 1987;72: 173-187.
Peter J, Burbach H, Adan RA, Lolait SJ, van Leeuwen FW, Mezey E, Palkovits M, Barberis C: Molecular neurobiology and pharmacology of the vasopressin/oxytocin receptor family. Cell Mol Neurobiol 1995;15: 573-595.
Seibold A, Brabet P.., Rosenthal W, Birnbaumer M: Structure và chromosomal localization of the human antidiuretic hormone receptor gen. Am J Hum Genet 1992;51: 1078-1083.
Sugimoto lớn T, Sailớn M, Mochizuki S, Watanabe Y, Hashimokhổng lồ S, Kawashima H: Molecular cloning and functional expression of a cDNA encoding the human V1b vasopressin receptor. J Biol Chem 1994;269: 27088-27092.
Lolait SJ, O"Carroll AM, Brownstein MJ: Molecular biology of vasopressin receptors. Ann N Y Acad Sci 1995;771: 273-292.
Lolait SJ, O"Carroll AM, Mahan LC, Felder CC, Button DC, Young WS, 3rd, Mezey E, Brownstein MJ: Extrapituitary expression of the rat V1b vasopressin receptor gene. Proc Natl Acad Sci USA 1995;92: 6783-6787.
Inouye ST, Kawamura H: Persistence of circadian rhythmithành phố in a mammalian hypothalamic "island" containing the suprachiasmatic nucleus. Proc Natl Acad Sci USA 1979;76: 5962-5966.
Shibata S, Liou S, Ueki S, Oomura Y: Influence of environmental light-dark cycle và enucleation on activity of suprachiasmatic neurons in slice preparations. Brain Res 1984;302: 75-81.
Ur E, Capstick C, McLoughlin L, Checkley S, Besser GM, Grossman A: Continuous administration of human corticotropin-releasing hormone in the absence of glucocorticoid feedback in man. Neuroendocrinology 1995; 61: 191-197.
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Received: July 13, 2016Accepted: December 01, 2016Published online: February 22, 2017Issue release date: May 2017

Number of Print Pages: 9Number of Figures: 4Number of Tables: 1

ISSN: 1015-8987 (Print) eISSN: 1421-9778 (Online)

For additional information: https://www.vhpi.vn/CPB


Aguilera G: Regulation of pituitary ACTH secretion during chronic stress. Front Neuroendocrinol 1994;15: 321-350.
Antoni FA: Hypothalamic control of adrenocorticotropin secretion: advances since the discovery of 41-residue corticotropin-releasing factor. Endocr Rev 1986;7: 351-378.
Kabir NN, Kazi JU: Grb10 is a dual regulator of receptor tyrosine kinase signaling. Mol Biol Rep 2014;41: 1985-1992.
Vale W, Rivier C, Brown MR, Spiess J, Koob G, Swanson L, Bilezikjian L, Bloom F, Rivier J: Chemical và biological characterization of corticotropin releasing factor. Recent Prog Horm Res 1983;39: 245-270.
Autelitano DJ, Lundblad JR, Blum M, Roberts JL: Hormonal regulation of POMC gene expression. Annu Rev Physiol 1989;51: 715-726.
Bale TL, Vale WW: CRF & CRF receptors: role in stress responsivity & other behaviors. Annu Rev Pharmacol Toxicol 2004;44: 525-557.
Abou-Samra AB, Harwood JPhường, Manganiello VC, Catt KJ, Aguilera G: Phorbol 12-myristate 13-acetate và vasopressin potentiate the effect of corticotropin-releasing factor on cyclic AMPhường production in rat anterior pituitary cells. Mechanisms of action. J Biol Chem 1987;262: 1129-1136.
Gillies GE, Linton EA, Lowry PJ: Corticotropin releasing activity of the new CRF is potentiated several times by vasopressin. Nature 1982;299: 355-357.
Aguilera G, Psi mê Q, Rabadan-Diehl C: Regulation of pituitary vasopressin receptors during chronic stress: relationship to corticotroph responsiveness. J Neuroendocrinol 1994;6: 299-304.
Levin N, Blum M, Roberts JL: Modulation of basal và corticotropin-releasing factor-stimulated proopiomelanocortin gen expression by vasopressin in rat anterior pituitary. Endocrinology 1989;125: 2957-2966.
Veldhuis JD, Iranmanesh A, Johnson ML, Lizarralde G: Amplitude, but not frequency, modulation of adrenocorticotropin secretory bursts gives rise to the nyctohemeral rhythm of the corticotropic axis in man. J Clin Endocrinol Metab 1990;71: 452-463.
Yamase K, Takahashi S, Nomura K, Haruta K, Kawashima S: Circadian changes in arginine vasopressin cấp độ in the suprachiasmatic nuclei in the rat. Neurosci Lett 1991;130: 255-258.
Jin X, Shearman LPhường, Weaver DR, Zylka MJ, de Vries GJ, Reppert SM: A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock. Cell 1999;96: 57-68.
Plotsky PM, Vale W: Hemorrhage-induced secretion of corticotropin-releasing factor-lượt thích immunoreactivity into the rat hypophysial portal circulation and its inhibition by glucocorticoids. Endocrinology 1984;114: 164-169.
Fink G, Robinson IC, Tannahill LA: Effects of adrenalectomy and glucocorticoids on the peptides CRF-41, AVP. and oxytocin in rat hypophysial portal blood. J Physiol 1988;401: 329-345.
Antoni FA: Vasopressinergic control of pituitary adrenocorticotropin secretion comes of age. Front Neuroendocrinol 1993;14: 76-122.
Mc CS, Brobeck JR: Evidence for a role of the supraopticohypophyseal system in regulation of adrenocorticotrophin secretion. Proc Soc Exp Biol Med 1954;87: 318-324.
Whitnall MH: Regulation of the hypothalamic corticotropin-releasing hormone neurosecretory system. Prog Neurobiol 1993;40: 573-629.
de Goeij DC, Kvetnansky R, Whitnall MH, Jezova D, Berkenbosch F, Tilders FJ: Repeated stress-induced activation of corticotropin-releasing factor neurons enhances vasopressin stores & colocalization with corticotropin-releasing factor in the median eminence of rats. Neuroendocrinology 1991;53: 150-159.
Deng Q, Riquelme D, Trinc L, Low MJ, Tomic M, Stojilkovic S, Aguilera G: Rapid Glucocorticoid Feedback Inhibition of ACTH Secretion Involves Ligand-Dependent Membrane Association of Glucocorticoid Receptors. Endocrinology 2015;156: 3215-3227.
Prickett TCR, Inder WJ, Evans MJ, Donald RA: Interleukin-1 potentiates basal và AVP-stimulated ACTH secretion in vitro–the role of CRH pre-incubation. Horm Metab Res 2000;32: 350-354.
Walker JJ, Spiga F, Waite E, Zhao Z, Kershaw Y, Terry JR, Lightman SL: The origin of glucocorticoid hooc môn oscillations. PLoS Biol 2012;10:e1001341.
Engler D, Pham T, Liu JP, Fullerton MJ, Clarke IJ, Funder JW: Studies of the regulation of the hypothalamic-pituitary-adrenal axis in sheep with hypothalamic-pituitary disconnection. II. Evidence for in vivo ultradian hypersecretion of proopiomelanocortin peptides by the isolated anterior và intermediate pituitary. Endocrinology 1990;127: 1956-1966.
Wersinger SR, Ginns EI, O"Carroll AM, Lolait SJ, Young WS, 3rd: Vasopressin V1b receptor knockout reduces aggressive behavior in male mice. Mol Psychiatry 2002;7: 975-984.
Makara GB, Mergl Z, Zelemãng cầu D: The role of vasopressin in hypothalamo-pituitary-adrenal axis activation during stress: an assessment of the evidence. Ann N Y Acad Sci 2004;1018: 151-161.
Tanoue A, Ito lớn S, Honda K, Oshikawa S, Kitagawa Y, Koshimizu TA, Mori T, Tsujimoto G: The vasopressin V1b receptor critically regulates hypothalamic-pituitary-adrenal axis activity under both bít tất tay and resting conditions. J Clin Invest 2004;113: 302-309.
Rivier J, Rivier C, Vale W: Synthetic competitive antagonists of corticotropin-releasing factor: effect on ACTH secretion in the rat. Science 1984;224: 889-891.
Olsen L, Knigge U, Warberg J: Gastrin-releasing peptide stimulation of corticotropin secretion in male rats. Endocrinology 1992;130: 2710-2716.
Suda T, Nakano Y, Tozawa F, Sumitomo T, Sakhổng lồ Y, Yamadomain authority M, Demura H: The role of corticotropin-releasing factor & vasopressin in hypoglycemia-induced proopiomelanocortin ren expression in the rat anterior pituitary glvà. Brain Res 1992;579: 303-308.
Jard S, Barberis C, Audigier S, Tribollet E: Neurohypophyseal hormone receptor systems in brain and periphery. Prog Brain Res 1987;72: 173-187.
Peter J, Burbach H, Adan RA, Lolait SJ, van Leeuwen FW, Mezey E, Palkovits M, Barberis C: Molecular neurobiology and pharmacology of the vasopressin/oxytocin receptor family. Cell Mol Neurobiol 1995;15: 573-595.
Seibold A, Brabet P.., Rosenthal W, Birnbaumer M: Structure & chromosomal localization of the human antidiuretic hormone receptor gen. Am J Hum Genet 1992;51: 1078-1083.
Sugimokhổng lồ T, Saito M, Mochizuki S, Watanabe Y, Hashimoto lớn S, Kawashima H: Molecular cloning & functional expression of a cDNA encoding the human V1b vasopressin receptor. J Biol Chem 1994;269: 27088-27092.
Lolait SJ, O"Carroll AM, Brownstein MJ: Molecular biology of vasopressin receptors. Ann N Y Acad Sci 1995;771: 273-292.
Lolait SJ, O"Carroll AM, Mahan LC, Felder CC, Button DC, Young WS, 3rd, Mezey E, Brownstein MJ: Extrapituitary expression of the rat V1b vasopressin receptor gene. Proc Natl Acad Sci USA 1995;92: 6783-6787.
Inouye ST, Kawamura H: Persistence of circadian rhythmicity in a mammalian hypothalamic "island" containing the suprachiasmatic nucleus. Proc Natl Acad Sci USA 1979;76: 5962-5966.
Shibata S, Liou S, Ueki S, Oomura Y: Influence of environmental light-dark cycle & enucleation on activity of suprachiasmatic neurons in slice preparations. Brain Res 1984;302: 75-81.


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Ur E, Capstiông xã C, McLoughlin L, Checkley S, Besser GM, Grossman A: Continuous administration of human corticotropin-releasing hooc môn in the absence of glucocorticoid feedbaông xã in man. Neuroendocrinology 1995; 61: 191-197.